Analytica 2010 in Munich

Analytica 2010 is taking place from the 23rd to 27th of March in Munich and YMC will be taking part.

We would be glad, if you would also plan a short stop on our booth where we will introduce our
latest product releases which are:

In addition to that we of course also have on display our full range of YMC HPLC columns, a broad
array of HPLC accessories, as well as our Kronlab glass columns for you.

Our product and sales specialists will be available to provide competent and firm advice.

Just stop by the YMC Europe stand and take a short break with a cup of coffee or cold drink and an
informative conversation.

You will find us in Hall A2, Stand no 313.

You still don’t have a ticket? Don’t worry!
Just call us on 0049 (0) 2064 427 227 to get your free ticket!

We are looking forward to your visit

NEW: YMC-Triart

Multi-layered hybrid material for HPLC

As shown by our past history, YMC has always been at the forefront of column technologies. Now, with YMC-Triart the aim has been to develop particles, which will set new benchmarks for column quality, stability and robustness. In order to succeed, a revolutionary micro-reaction technology derived from “Flow Chemistry” has been developed. This allows YMC Triart not only to have the desired chemical composition, but also the reproducible particle properties of size, shape and reproducibility associated with robustness and outstanding column quality!

Why not try YMC-Triart today!

Do you spend too much time with scaling up your analyses?

In the era of UPLC and UHPLC, short analysis times are no longer a challenge. However, the step from analytical scale to the semi-preparative or preparative purifi cation can be associated with a time-consuming
method optimisation, especially as only a few column materials are fully scalable.

YMC has always committed to make the scale-up process as easy as possible by using fully scalable materials. The separation of fl avonoids from a Ginko extract demonstrates the successful scaleup process perfectly.

The YMC-UltraHT 2μm column was used for the analytic separation developed for the UPLC or UHPLC.
The large scale purifi cation took place with a semi-preparative high-throughput column YMC-Actus,
packed with 5μm material.

Specific characteristics of YMC phases: all particle sizes are manufactured in a standardised production procedure, so no method optimisations is necessary!

Fraction 1 (Peak 1 = Quercetin, 100% purity)

Fraction 2 (Peak 2 = Kaempferol, 100% purity)

Fraction 3 (Peak 3 = Isorhamnetin, 95% purity)

Surviving the CIP cycle with YMC BioPro IEX media

Stability of BioPro IEX material under CIP conditions

CIP-ing, thus performing cleaning in place is an important part of any pharmaceutical production process. For this reason, normally, all production equipment is designed in such a way that it can be cleaned completely and verifiably, without the need to dismantle it.

It goes without saying that the chromatographic media also have to comply to this requirement and that they have to be able to withstand the harsh conditions of the CIP-cycles. It is expected that after the CIP cycle they perform the same way they performed upon first usage. Therefore the IEX material YMC BioPro SP 75 μm was tested with regards to its ability to withstand the CIP procedure. As a result it was found that even after 20 CIP-cycles the material showed the same high values for dynamic binding capacity (DBC), recovery and separation that made it a high class material in the first place. A schematic drawing of the testing cycle is given in figure 1. During one cycle, first a chromatogram for the separation of three standard proteins was obtained. Next the DBC and recovery for Lysozyme were determined, before the medium was "CIP’ed" with 1M NaOH for 5 CV at a linear flow of 400 cm/h.

The protein mixture, used for the separation was made up of Ribonuclease A, Cytochrom C and Lysozyme. These were separated at a flow rate of 180 cm/h using a 20 mM potassium phosphate buffer pH 6.8 as mobile phase A and 20 mM potassium phosphate buffer pH 6.8 containing 0.5M NaCl as phase B. The dynamic binding capacity for Lysozyme was determined at 10% breakthrough.
For this, the column was loaded with Lysozyme in 20 mM Glycin-NaOH buffer pH 9.0 at a linear flow of 800 cm/h. For the measurement of the recovery 0.5M NaCl was added to the same buffer system (without Lysozyme).

In figure 2 the DBC and recoveries are plotted after each CIP step. For every cycle the DBC stays at a very good value of generally above 220 mg Lysozyme per ml resin. Also the recovery remains unchanged with generally 100%.

Finally, figure 3 shows that there is no difference in the ability to separate the protein mixture between the various runs.

As a result it can be stated that the usage of 5 CV 1M NaOH at a flow rate of 400 cm/h is a
suitable CIP-procedure. Furthermore, the experiments show that the YMC BioPro SP 75 μm material withstands at least 20 such CIP cycles, while keeping its high class properties for DBC, recovery and separation power.

YMC BioPro IEX materials are, therefore, well suited for usage in big biopharmaceutical downstream processes. The resins are available as strong cation exchanger (YMC BioPro SP) and strong anion exchanger (YMC BioPro QA), both in particle sized of 30 μm and 75 μm.

For any further questions regarding „cleaning in place“ of our materials, or to obtain a sample of one of these materials, please contact:

Binary prep. HPLC-system for processing large volumes of ureacontaining protein solutions

Often client specific requirements for multipurpose equipment can not be solved with “off the shelf“ solutions. In this specific case, the Kronlab custom build approach proved very useful: every component has been specifically approved for the application at hand and following a specified procedure it
is integrated into an LC system. The result is
a made-to-measure system.

In this example, which has been designed
for a small to medium scale production
process, the challenge was to introduce
large volumes of sample, which contained
proteins dissolved in urea, onto a reversed
phase system. The answer was to implement a “metal-free” HPLC pump, through which the sample could be pumped directly onto the column, using a 4-way 2- position valve. Obviously the size and quality of the pump was chosen in such a way that there was no need to compromise on the speed at which the sample is introduced to the system, compared to the normal flow rate of the system. As this pump can be completely integrated into the system software, the process can be fully automated.

Additionally the use of a diode array UV detector enables simultaneous data acquisition at multiple wavelengths. The inclusion of a software controlled 8 port fraction collector makes it possible to collect fractions based on peak slope, threshold and time to ensure only genuine sample peaks are selected.

Finally Kronlab supplies the customer with detailed documentation of testing as well as qualification or support for IQ/OQ/PQ verification. Individual, client-specific testing and acceptance criteria can be incorporated on request.

Maintenance and service is provided by qualified scientists or skilled engineers, all with many years of experience with such systems.

Therefore when a “made to measure” solution is needed, contact:- Kronlab – the engineering division of YMC Europe GmbH.

Glass columns – not only for biochromatography

In the laboratory glass columns are favoured by many chromatographers because of their inertness towards proteins. Also the possibility to clean the packed columns with highly alkaline caustic soda solutions, a widely-used purification step, is a good argument for glass columns.
While the suitability of glass columns for biochromatography is well-known, the use in NP- or RP-chromatography with silica based stationary phases is rarely considered. The main reason maybe the concern that the high back pressure of 15μm or smaller silica particles may exceed the specifications of the column. However glass columns that withstand high back pressures from 30 (for 50mm i.d) up to 80bar (for 5mm i.d.) are available. These allow most demanding separations with long silica chromatographic beds or high flow rates for fast elution.
The figure shows the separation of a standard sample, using different flow rates, to illustrate the enormous time saving of 75% that is made possible by these greater pressure ratings.

Photochemistry in a micro reactor: LUMINO from YMC

It is the combination of micro technology and high performance LED’s which make this product so valuable and special.

The typical applications for photo reactors are mainly photo chemical steps in the pharmaceutical and biotechnology industry, as well as some selected area in other industries. The technology platform of photo reactors can make for an efficient way for the photochemical synthesis of active
pharmaceutical ingredients (API) as well as the photo-bio-production of API’s and substances for the cosmetic industry, through employing algae or bacteria.

Contrary to conventional, thermal methods, with photo bioreactors the energy needed for the reaction can be supplied in a relatively mild way.

Another important advantage of photochemical production arises from the selectivity of the light induced chemical or biological process. This often leads to highly pure products.

As a light sources high performance LED’s of various wavelength are available. The system features temperatures in the region of 20 – 160°C and comes with a TiO2 coated micro reactor. Many different reactors and accessories are available.

We are happy to provide you with additional and “in depth” material. Furthermore, we offer to explain the benefits of the system for your application in personal conversation.