New: YMC-Columns for SFC!

Efficient separation of achiral APIs in SFC mode

In recent years, SFC has gained enormously in importance and acceptance in the pharmaceutical industry. Nowadays, it is routinely used for the purification of enantiomers. In addition, SFC has assumed a relevant role both in analytical separations and larger scale purification of achiral compounds as it is seen as an economic and “green” alternative to classical HPLC.

• Less solvent consumption
  The use of supercritical CO2 as the main component of the mobile phase drastically reduces the consumption of expensive and environmentally harmful organic solvents. In SFC mode, an average of about 80% less organic solvent is required than in classical normal phase (NP) HPLC.


• Faster separations & higher throughput
  Because of the low viscosity and high diffusibility of the supercritical mobile phase, SFC separations are at least 3-5 times faster than in classical HPLC. As a result, sample throughput in SFC mode is very high, which reduces the time pressure in the pharmaceutical industry.


• Wide range of selectivities for achiral SFC
  YMC-Diol, YMC-Amino, YMC-Cyano, YMC-PVA-Sil, YMC-Polyamine II and YMC-Silica provide a broad range of different SFC selectivities which can solve the most diverse separation problems.


• Special SFC column hardware
  For preparative SFC YMC has developed special column hardware in order to meet the highest demands in quality, safety and reliability. YMC SFC columns are available in a number of lengths with 20, 30 or 50 mm I.D.
  

(YMC SFC column 250 x 50 mm I.D.)

SFC Selectivity Study

Retention characteristics and selectivity of six achiral HPLC phases from YMC were tested in SFC mode
with a generic SFC gradient (*):

1 Citral 2 Lidocaine 3 Caffeine 4 Ibuprofen 5 Amitriptyline 6 Propranolol 7 Procainamide 8 Sulpiride 9 β-D-Arabinofurano-sylcytosine 10 Sulfinpyrazon

(*) All measurements were done at Averica Discovery Services, Inc., One Innovation Drive, Three Biotech, Worcester, MA 01605 USA.

The stationary phases studied show significant differences in selectivity in SFC mode, including reversal of elution order of some of the components. Therefore a phase screening process, similar to that used in reversed phase chromatography, using a generic SFC gradient is recommended to find the optimum phase.

Our tip: YMC PVA-SIL
YMC PVA-SIL is a general purpose phase for SFC separations. It gives very sharp peaks and high selectivity for all the components in the sample, making it the phase of choice for acidic, basic and neutral compounds.

Ordering information:

Further information on these stationary phases, column dimensions and bulk media for SFC are available on request.

Often client-specific requirements for multipurpose equipment can not be solved with “off-the-shelf” solutions. In this specific case, Kronlab’s custom-build approach proved very useful: every component has been specifically approved for the application required and following a specified procedure it is integrated into an LC system. The result is a made-to-measure system.

This example has been designed for method optimisation of peptide separations in pilot scale and the intended use in a potentially explosive atmosphere.
In addition to a binary high pressure gradient pump and a “metal-free” HPLC pump for sample application, modules for temperature, mass flow and pressure data acquisition were included. A 4-way 2-position valve enables the bypassing of the column. A multiple wavelength UV detector ensures the detection of the target peptides. The fractionation of up to 32 samples works with a well-thought-out valve assemble which allows the purging of the fractionation tubing with nitrogen after fraction change and is not limited to a defined volume.

The careful choice of components and their placing in a nitrogen pressurised cabinet allows work in areas with potentially explosive atmosphere to be undertaken safely.

Finally Kronlab provides the customer with detailed documentation of testing as well as qualification or support for IQ/OQ/PQ verification. Individual, client-specific testing and acceptance criteria can be incorporated on request.

Maintenance and service is provided by qualified scientists or skilled engineers, all with many years of experience with such systems.

Therefore when a “made-to-measure” solution is needed, contact:

Kronlab – The Engineering Division of YMC Europe GmbH

Your direct contact: Lars Henning, E-Mail: henning@ymc.de

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