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YMC-Actus for fast semi-preparative HPLC high throughput separations

Overview Properties Applications Literature   Excellent durability and efficiency under fast gradient condition at high flow rate Outstanding separation of highly polar compounds Excellent separations of compounds with similar structure Excellent durability and efficiency under fast gradient condition at high flow rate Glycyrrhizic acid in herb medicine Eluent: A) water/acetic acid (99/1) B) methanol/acetic acid (99/1) 20%B (0-2min), 20-45%B (2-7min), 45%B (7-10min) Temperature: ambient Detection: UV at 250 nm Sample: water/methanol/acetic acid extract of commercially available herb medicine (0.1g/ml)   Outstanding separation of highly polar compounds Crude synthetic 30mer oligonucleotide Eluent: A) 10mM DBA-acetic acid (pH6.0)/methanol (60/40) B) 10mM DBA-acetic acid (pH6.0)/methanol (20/80) 10-35%B (0-20min) Temperature: ambient Detection: UV at 269 nm Sample: synthetic oligonucleotide (100µm) Excellent separations of compounds with similar structure Capsaicinoids in red pepper Eluent: A) methanol/water/TFA (50/50/0.1) B) methanol/TFA (100/0.1) 0-30%B (0-5min), 30%B (5-10min) Temperature: 25°C for 50 x 4.6 mm i.d. ambient for 50 x 20 mm i.d. Detection: UV at 280 nm Sample: methanol extract of a commercial red pepper (1g/3ml)