Rapid pressure changes under high-speed
gradient conditions may lead to column degradation
and loss of column performance.
With YMC-Actus, a new hardware and packing
technology has been applied to these
semi-preparative columns to provide a uniform
packing density which results in a longer
lifetime than conventional semi-preparative
columns.
In order to determine the quality of the packing,
the column performance was evaluated
every 100 runs with a sequential high
throughput gradient.
YMC-Actus columns offer outstanding efficiency
without compromising resolution.
Furthermore, YMC-Actus columns provide
reliable results, even after exposure to severe,
rapid gradient conditions and multiple
injections. |
|
| |
Sequential gradient test |
| |
Column: |
50 x 20 mm i.d., 5 μm
or 50 x 19 mm i.d., 5 μm |
| |
Eluent: |
A) water B) methanol |
| |
Gradient: |
5% B (0-0.5 min), 5-95% B (0.5-3.1 min),
95% B (3.1-3.6 min), 5% B (3.6-4.0 min) |
| |
Flow rate: |
50 ml/min |
| |
Temperature: |
ambient |
| |
Column performance test |
| |
Column: |
50 x 20 mm i.d., 5 μm
or 50 x 19 mm i.d., 5 μm |
| |
Eluent: |
methanol / water (70/30) |
| |
Flow rate: |
10 ml/min |
| |
Sample: |
Naphthalene |
|
Start
