• Hydrophilic Interaction Chromatography (HILIC)
• Applications
• Literature

Advantages

In general, reliable applications in HPLC require a minimum retention factor (k’) of 2. In the widely
used reversed phase methods polar compounds such as peptides, organic acids or pyrimidines
often exhibit little or no retention and therefore low resolution on a conventional C18 phase. The
use of additives such as ion-pair reagents is not an alternative since it is not applicable to LC-MS.
Also normal phase chromatography fails due to poor solubility of the polar compounds in organic
solvents. HILIC fi lls the gap between reversed phase and normal phase chromatography, because
polar compounds can be retained while using e.g. acetonitrile/water eluents and buffers suitable for
LC-MS.