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Analytical Columns
YMC Ultra Fast LC Columns
 
 
 
 
 
 

 
Ultra Fast LC Columns
 

Melamine in pet-food

Sample preparation method

Melamine in Pet Food

Melamine in Pet Food Application
Column:
Part No.:
Eluent:

Gradient:
Flow rate:
Temperature:
Detection:
Injection: 		YMC-UltraHT Hydrosphere C18 (2 μm, 12 nm) 50 x 2.0 mm ID
HS12S020502WT
A) water / heptafluorobutyric acid (100/0.1)
B) methanol / heptafluorobutyric acid (100/0.1)
5% B (0-0.17 min), 5-90% B (0.17-3 min)
0.4 ml/min
40 °C
UV at 240 nm
1 μl

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Curcuminoids in a commercial turmeric supplement

Curcuminoids Application A

Curcuminium Application B

 
Column:
Part No.:
Eluent:
Flow rate:
Temperature:
Detection:
Injection:

Sample:
 YMC-UltraHT Pro C18 (2 μm, 12 nm) 100 x 2.0 mm ID
AS12S021002WT
acetonitrile/water/formic acid (50/50/0.1)
0.4 ml/min
40 °C
A) UV at 250 nm, B) UV at 420 nm
Standard 1 μl (0.1 mg/ml)
Supplement 1 μl (6.9 mg/ml)
methanol extract of a commercially available turmeric supplement

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Separation of water-soluble vitamins

Separation of water-soluble vitamins

Column:
Part No.:
Mobile Phase:

Gradient:






Flow rate:
Detection:
Pressure:
Injection:
Temperature:

YMC-UltraHT Hydrosphere C18 (12 nm, 2 μm)50 x 2.0 mm ID
HS12S020502WT
A: acetic acid (pH 3.5)
B: acetonitrile / acetic acid (pH 3.5) (20/80)
Time A (in %) B (in %)
0.0 10 90
0.2 0 100
1.0 0 100
1.2 10 90
2.0 10 90

0.76 ml/min
210 nm
450 bar
1 μL
40 °C

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Aromatic carboxylic acids in foods

Applications Aromatic carboxylic acids in foods
Column:
Part No.:
Eluent:



Flow rate:
Temperature:
Injection:
YMC-UltraHT Pro C18 (2 μm,12 nm) 50 x 2.0 mm I.D.
AS12S020502WT
A) water/HCOOH (200/0.4)
B) acetonitrile/methanol/THF/water/HCOOH
(43.5/43.5/13/100/0.4)
20-100%B (0-2.8 min)
0.5 ml/min
40 °C
2 μl (2.530 μg/ml)

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Separation of paracetamol and impurities

Application Separation of paracetamol and impurities

Column:
Part No.:
Mobile Phase:

Gradient:




Flow rate:
Detection:
Pressure:
Injection:
Temperature:

YMC-UltraHT Pro C18 (2 μm, 12 nm) 75 x 2.0 mm ID
AS12S02L502WT
A: acetonitrile
B: formic acid (pH 2.8)
Time A (in %) B (in %)
0.0 42 58
0.1 42 58
0.5 80 20
1.5 80 20
0.5 ml/min
UV at 254 nm
420 bar
1 μl
40 °C

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Separation of sulfa drugs

Application Separation of sulfa drugs

Column:
Part No.:
Mobile Phase:

Gradient:
 
 
 
 

Flow rate:
Detection:
Pressure:
Injection:
Temperature:

YMC-UltraHT Hydrosphere C18 (2 μm,12 nm)75 x 2.0 mm ID
HS12S02L502WT
A: methanol
B: formic acid (pH 2.5)
Time A (in %) B (in %)
0.0 18 82
0.1 18 82
2.8 42 56
2.9 100 0
4.0 100 0

0.45 ml/min
UV at 280 nm
460 bar
1 μl
40 °C

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Separation of seven analgenics

Separation of seven Alagenics

Column:
Part No.:
Mobile Phase:

Gradient:
 
 
 

Flow rate:
Detection:
Pressure:
Injection:
Temperature:

YMC-UltraHT Hydrosphere C18 (2 μm, 12 nm)50 x 2.0 mm ID
HS12S020502WT
A: acetonitrile
B: formic acid (pH 2.5

Time A (in %) B (in %)
0.0 25 75
1.0 90 10
1.2 90 10

0.8 ml/min
UV at 240 nm
350 bar
μl
40 °C

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Separation of pain killing drugs

Application Separation of pain killing drugs

Column:
Part No.:
Mobile Phase:

Gradient:
 
 
 

Flow rate:
Detection:
Pressure:
Injection:
Temperature:

YMC-UltraHT Pro C18 (2 μm, 12 nm) 50 x 2.0 mm ID
AS12S020502WT
A: methanol; B: formic acid pH (0.15%)

Time A (in %) B (in %)
0.0 62 38
0.2 62 38
0.5 100 0
1.5 100 0
2.0 62 38

0.5 ml/min
UV at 254 nm
390 bar
1 μl
40 °C

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