YMC30 columns are successfully used in the food industry, for the analysis of vitamin formulations, in environmental analysis, and for the control of the growth of algae. Other potential applications include the separation of prostaglandins or leucotrienes.

Extract of SRM 2383, NIST food standard A

Column: YMC30, 250 x 4.6 mm i.d. Eluent: A: MeOH/MTBE/H2O = 81/15/4 , B: MeOH/MTBE/H2O = 6/90/4 Gradient: 100/0 - 0/100 in 90 min Flow: 1.0 ml/min Detection: UV at 450 nm Temperature: ambient

Carotene and Xanthophyll standard A

Column: YMC30, 250 x 4.6 mm i.d. Eluent: A: MeOH/MTBE/H2O= 81/15/4 , B: MeOH/MTBE/H2O= 6/90/4 Gradient: 1-100 % B (90 min) Flow: 1.0 ml/min Detection: UV at 450 nm Temperature: ambient

Carotenoid pigments in algae B

Column: YMC30, 250 x 4.6 mm i.d. Eluent: A: MeOH/acetone = 60/40, B: acetone/H2O = 60/40 Gradient: 60-30 % B (0-3 min), 30 % B (3-22 min), 30-10 % B (22-26 min), 10 % B (26-41.5 min), 10-60 % B (41.5-45 min) Flow: 0.5 ml/min Detection: UV at 450 nm Temperature: 35 ºC

Column care
Good separations have been achieved with typical non-aqueous reversed phase mobile phases. To prevent a loss in selectivity by the reversible folding of C30 chains, a high water content of the eluent has to be avoided. The recommended pH range for using YMC30 columns is 2-6. Remove acid and buffer salts before storage. Store the column in 100% methanol. Clogged inlet frits can be cleaned by changing the flow direction or replacement.
For detailed information please refer to the “Column Care and Use Instructions”, which are shipped with each analytical column.