| Elution profile of complexing agents |
|
Column:
Eluent:
Flow:
Detection:
Temperature:
|
J’sphere ODS-H80 (8 nm, 4 μm) 150 x 4.6 mm i.d.
K2HPO4-KH2PO4 (20 mM, pH 6.9) / methanol = 40/60
1.0 ml/min
UV at 254 nm
37 °C
|
|
|
| Triazine herbicides |
|
Column:
Eluent:
Flow:
Detection:
Temperature:
|
J’sphere ODS-H80 (8 nm, 4 μm)
150 x 4.6 mm i.d.
acetonitrile / NH4H2PO4 (50mM) = 45/55
1.0 ml/min
UV at 230 nm
37 °C
|
|
| Thiamine and derivatives |
|
Column:
Eluent:
Gradient:
Flow:
Detection:
Temperature:
|
J’sphere ODS-H80 (8 nm, 4 μm) 75 x 4.6 mm i.d.
A: (NH4)2HPO4 (50 mM)
B: methanol / (NH4)2HPO4 (50 mM) = 60/40
10-100% B (0-15 min, linear), 100% B (15-30 min)
1.0 ml/min
UV at 260 nm
37 °C
|
|
|
| Urea herbicides |
|
Column:
Eluent:
Flow:
Detection:
Temperature:
|
J’sphere ODS-H80 (8 nm, 4 μm)
150 x 4.6 mm i.d.
THF / H2O = 30/70
1.0 ml/min
UV at 260 nm
37 °C
|
|
Applications
J’sphere ODS-H80
J’sphere ODS-H80 is the most hydrophobic stationary phase in this series. It is densely covered with
polymeric bonded C18 groups yielding a high carbon content and providing a strong, dominant,
lipophilic interaction with the nonpolar sites of the sample molecules. However, the ability to form
H-bonding gives additional selectivity, which is essential for difficult separations, such as drug and
corresponding metabolite discrimination. Even stereoisomers can be separated by J’sphere ODSH80
columns.
J’sphere ODS-M80
The lower coverage of C18 monomeric bonded groups in J’sphere ODS-M80 provides moderate
hydrophobicity. As the lipophilic character is decreased, the H-bonding capacity becomes more and
more important. J’sphere ODS-M80 has a pronounced balanced polarity which is extraordinary flexible
and allows application to a wide variety of separation problems. Depending on the separation,
J’sphere ODS-M80 columns can be operated over a very broad range of eluent polarity. J’sphere
ODS-M80 columns are a very adaptable tool in various fields in analytical HPLC including drug
analysis and QC.
J’sphere ODS-L80
J’sphere ODS-L80 has a low polymeric bonded C18 coverage, providing only minor hydrophobic
retention. The extremely high H-bonding capacity makes J’sphere ODS-L80 very useful for the separation
of polar compounds. Such compounds frequently have molecular sites which are susceptible
to H-bonding and hence, are easily separated by a H-bonding mechanism.
Conclusion
By using the graduated properties of J’sphere ODS columns, a great variety of chemical and pharmaceutical applications can be achieved. YMC J’sphere ODS analytical columns are a good choice for the analysis of pharmaceuticals, organic intermediates, metabolites etc., due to their concept of fine-tuned approach by using different H-bonding capacities.
Quality Specifications
Based on the experience in high performance analytical selectivities and large scale silicas synthesis
and bonded phases, the long term availability of high quality analytical J’sphere ODS columns is
guaranteed. Sophisticated selectivity tests for quality control ensure reproducible separations. These
quality control tests guarantee the customer long term reproducible performance, which is essential
for the validated analytical HPLC methods.
|
Start
