| 2,4-Dinitrophenylhydrazones of aldehydes
and ketones |
|
Column:
Eluent:
Flow:
Detection:
Temperature:
Injection: |
YMC-Pack C4 (5 μm ,12 nm) 150 x 4.6 mm i.d.
A = tetrahydrofuran / water (10/90) B = acetonitrile; 35%
B (0-7 min), 35-65% B (7-18 min, linear),
100% B (18-19 min), 35% B (19-35 min)
1.5 ml/min
UV at 360 nm, 0.01 AUFS
30 °C
11 μl (0.0025 mg/ml) |
| Anti-human immunodeficiency virus (HIV) agents |
|
Column:
Eluent:
Flow:
Detection:
Temperature:
Injection: |
YMC-Pack C4 (5 μm, 12 nm) 150 x 4.6 mm i.d.
methanol / 10 mM KH2PO4 (10/60)
1.0 ml/min
UV at 254 nm, 0.16 AUFS
37 ºC
7 μl (0.125 mg/ml) |
| Proteins |
|
Column:
Eluent:
Flow:
Detection:
Temperature:
Injection: |
YMC-Pack C4 (5 μm, 30 nm) 150 x 4.6 mm i.d.
A) acetonitrile / water / TFA (5/95/0.1)
B) acetonitrile / water / TFA (60/40/0.1)
1.0 ml/min
UV at 220 nm, 0.32 AUFS
37 ºC
16 μl (0.16 ~ 0.33 mg/ml) |
| Water-soluble vitamins |
|
Column:
Eluent:
Flow:
Detection:
Temperature:
Injection: |
YMC-Pack C4 (5 μm, 12 nm) 150 x 4.6 mm i.d.
acetonitrile / 50 mM NH4H2PO4 (10/90)
1.0 ml/min
UV at 210 nm, 0.16 AUFS
37 ºC
10 μl (0.02 ~ 0.30 mg/ml) |
|
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