YMC-Pack C4 packings are less hydrophobic and generally require more aqueous buffer than C8 or C18 packings. When compared to C8 or C18 packings using the same eluent, YMC-Pack C4 shows significantly shorter retention times for nonpolar compounds. Retention of polar compounds, however, is not significantly affected. Therefore, mixtures with a wide range of component polarity are best separated by YMC-Pack C4. This is because the butyl bonded phase gives shorter retention times while still maintaining high resolution when compared to longer chain bonded chemistries as demonstrated in the figure below.

Column care
YMC-Pack C4 is stable towards hydrolysis between pH 2-7,5. Remove acid and buffer salts before storage. Store the column in methanol / water = 70/30. Clogged inlet frits often can be cleaned by changing the flow direction or replacement.
For detailed information please refer to the “Column Care and Use Instructions”, which are shipped with each analytical column.