| Nucleotides |
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YMC-Pack ODS-AM (5 μm, 12 nm) 250 x 4.6 mm i.d.
A = 0.2 M triethylamine-acetic acid (pH 6.6)
B = 0.2 M triethylamine-acetic acid (pH 6.6) / ACN (95/5)
4% B (0-10 min), 4-100% B (10-35 min, linear),
100% B (35-50 min)
UV at 260 nm, 0.16 AUFS
1.0 ml/min
5 μl (0.27 ~ 0.71 mg/ml)
30 °C
14 µl (0.029 ~ 0.16 mg/ml)
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| PTC-amino acids |
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YMC-Pack ODS-AM (5 μm, 12 nm) 250 x 4.6 mm i.d.
A = 60 mM CH3COONa - CH3COOH (pH 6.6) / ACN (94/6)
B = 60 mM CH3COONa - CH3COOH (pH 6.6) / ACN (40/60)
0-55% B (0-20 min, linear),
100% B (20-25 min), 0% B (25-35 min)
1.0 ml/min
UV at 250 nm, 0.08 AUFS
37 °C
5 μl (62.5 pmol/μl)
PTC derivatives |
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