| Estrogens and progestins |
 |
Column: YMC-Pack C8 (Octyl) (5 μm, 12 nm) 250 x 4.6 mm i.d.
Eluent: acetonitrile / THF / water (46/4/50)
Flow: 0.7 ml/min
Detection: UV at 230 nm, 0.16 AUFS
Temperature: 30 ºC
Injection: 7 μl (0.1 mg/ml) |
| Estrogens and progestins |
 |
Column: YMC-Pack C8 (Octyl) (5 μm, 12 nm) 150 x 4.6 mm i.d.
Eluent: acetonitrile / 20 mM KH2PO4 (5/95)
Flow: 1.0 ml/min
Detection: UV at 260 nm, 0.64 AUFS
Temperature: 30 ºC
Injection: 13 μl (0.2 mg/ml) |
| Resorcylic acid isomers |
 |
Column: YMC-Pack C8 (Octyl) (5 μm, 12 nm) 150 x 4.6 mm i.d.
Eluent: methanol / 100 mM KH2PO4 (10/90)
Flow: 0.8 ml/min
Detection: UV at 285 nm, 0.16 AUFS
Temperature: 37 ºC
Injection: 10 μl (0.05 ~ 0.4 mg/ml) |
|
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