| Analysis of Potato Lipids |
|
Column:
Flow rate:
Mobile Phase: |
YMC-Pack PVA-Sil (5 μm, 12 nm) 250 x 4.6 mm i.d.
1 to 2 ml/min
A: iso-hexane / methyl ter butyl ether (98:2,v/v)
B: propan-2-ol / ACN / CHCl3 / CH3OOH (84:8:8:0.025)
C: propan-2-ol / water / triethylamine (50:50:0.2,v/v) |
Gradient:
Tmin:
B%:
C%:
Flow (ml/mn): |
0
0
0
1 |
5
20
0
1 |
15
52
4
1 |
40
52
14
1,4 |
40,1
70
0
2 |
45
0
0
2 |
50
0
0
2 |
|
| Nebuliser temperature: 25 °C, Evaporation temperature: 35 °C |
S:
MGDG:
PE:
PC:
PI:
CER: |
Sterols SE: Sterol Esters
Monogalactosyldiacylglycerols
Phosphatidylethanolamine
Phosphatidylcholine
Phoasphatidylinositol
Cerebrosides |
SG:
DGDG:
PG:
ASG:
DG: |
Steryl glycosides
Digalactosyldiacylglycerols
Phosphatylglycerols
Acylsterylglycosides
Diacylglycerol |
| Uracil (in HILIC-mode) |
|
Column:
Eluent:
Flow rate:
Detection: |
YMC-Pack PCA-Sil (5 μm, 12 nm) 100 x 3.0 mm i.d.
acetonitrile / CH3COONH4; 200 mM, pH 5,5
isocratic (95/5)
0.9 ml/min
UV at 275 nm |
| Analysis of Lipids |
|
Column:
Eluent:
Flow rate:
Detection: |
YMC-Pack PVA-Sil (5 μm, 12 nm) 250 x 4.0 mm i.d.
A: n-hexane / tert-methylbutyl ether (98:2, vol/vol)
B: isopropanol / acetonitrile / chloroform / acetic acid ( 84:8:8:0.025, v/v)
C: isopropanol / water / triethylamine (50:50:0.2, by vol)
plus 5 mM ammonium sulfate
1 ml/min
ELSD |
| |
|
|
|
SE:
TG:
Sterol:
SQDG:
DHCer:
Cer:
DGDG: |
steryl oleate
TAG/tripentadecanoin
stigmasterol/sitosterol
sulfoquinovosyldiacylglycerol
dehydroxycerebroside
cerebroside
digalactosyldiacylglycerol |
PE:
PA:
PC:
SPH:
LPC: |
PE-dipalmitoyl
PA-diheptadecanoyl
PI, PS, PC-diheptadecanoyl
sphingomyelin
lysophosphatidylcholine |
|
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