Normal phase alternative to Silica
PVA-Sil, which possesses a polyvinyl alcohol (PVA) surface chemistry, is an excellent alternative to silica gel or other polar bonded phases which are used in normal phase chromatography. In many situations it exhibits better performance characteristics and a unique selectivity and can often resolve compounds that behave poorly on silica. The alcohol functionality present on PVA-Sil is better suited for troublesome compounds, such organic bases, than acidic silanols present in unbonded silica.
Since PVA-Sil is a bonded stationary phase, it can be washed with solvents of any polarity, from hexane through water, without altering the surface activity. Therefore selectivity, retention and resolution are reproducible regardless of the column’s previous history. This is not true of bare silica, which easily becomes completely deactivated following the introduction of even small quantity of water.
Provides high sample recovery
The surface of PVA-Sil is very uniform without the highly active acidic silanol sites on bare silica which can cause decomposition of sensitive molecules. Because of consistent surface activity, PVA-Sil exhibits neither non-specific irreversible adsorption nor sample degradation. This is a problem often encountered with bare silica columns. The lack of non-specific adsorption and the uniformity of the polyvinyl alcohol bonded surface means that, unlike silica, PVA-Sil can be reused over and over without fear of contamination or carryover. Sample recoveries on PVA-Sil typically average 90% or higher.
Excellent choice for packed column SFC
The PVA polymer shell on PVA-Sil deactivates the silica support while providing a hydrophilic surface. This, coupled with available column dimension of 1.0 mm and 2.0 mm i.d. means that PVA-Sil columns are well suited for SFC separations.
Column care
YMC-Pack PVA-Sil is stable towards hydrolysis between pH 2-9,5. Remove acid and buffer salts before storage. Store the column in methanol / water = 70/30. Clogged inlet frits often can be cleaned by changing the flow direction or replacement.
For detailed information please refer to the “Column Care and Use Instructions”, which are shipped with each analytical column.
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