| Peptides and proteins |
 |
Column: YMC-Pack PolymerC18 150 x 4.6 mm i.d.
Eluent: A = acetonitrile / water / TFA (20/80/0.05)
B = acetonitrile / water / TFA (45/55/0.05)
0-100% B (0-30 min, linear)
Flow: 1.0 ml/min
Detection: UV at 220 nm, 0.32 AUFS
Temperature: 30 ºC
Injection: 30 μl |
| Aminoalcohols |
 |
Column: YMC-Pack PolymerC18 250 x 6.0 mm i.d.
Eluent: 100 mM Na2HPO4 / 100 mM NaOH (60/40, pH 12.0)
Flow: 0.6 ml/min
Detection: UV at 215 nm, 0.32 AUFS
Temperature: 20 °C
Injection: 50 μl (0.2 ~ 3.0 mg/ml) |
| Organic acids |
 |
Column: YMC-Pack PolymerC18 250 x 4.6 mm i.d.
Eluent: 0.1% TFA
Flow: 0.5 ml/min
Detection: UV at 220 nm, 0.08 AUFS
Temperature: 30 °C
Injection: 10 μl (0.016 ~ 2.2 mg/ml) |
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