The proprietary derivatisation procedure for YMCbasic allows YMC to produce a material with controlled surface coverage, which shows excellent lot-to-lot reproducibility as a result of closely monitoring both the production of the silica support and the bonding process.

Hydrophobicity of YMCbasic versus YMC-Pack Octyl and YMC-Pack ODS-AM Column: 250 x 4.6 mm Eluent: acetonitrile / water (75/25) Flow: 1.0 ml/min Detection: UV at 254 nm

The resulting YMCbasic material shows a different hydrophobicity compared with C8 or C18 phases as shown in the diagram. In addition, it also represents an interesting alternative to short chain selectivities.

Applications
The unique chemistry provides a phase with true reversed phase characteristics, high resolution and excellent peak symmetry for basic compounds without the need for ion pair reagents or amine modifiers (see separation of anilines using acetonitrile /water eluent). Unlike many base-deactivated phases, YMCbasic is also suitable for separation of acidic compounds, showing slight retention of highly polar acid compounds such as maleate. YMCbasic provides a complementing selectivity seen with conventional C8 and C18 materials, but without peak tailing for basic compounds.

Column care
The recommended pH range for YMCbasic is 2-7,5. Remove acids and buffer salts before storage. Store the column in methanol/water=70/30. Clogged inlet frits often can be cleaned by changing the flow direction or replacement.
For detailed information please refer to the “Column Care and Use Instructions” which are shipped with each analytical column.