Finally, Hydrosphere C18 is, in common with all ProFamily members, applicable to LC-MS methods as shown below. High throughput LC-MS separations with enhanced flow rates easily achievable with 3 µm version of Hydrosphere C18
| LC-MS compatibility |
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Column: Hydrosphere C18 (3 µm, 12 nm) 100 x 2.0 mm i.d.
Eluent: 10 mM CH3COOH - CH3COONH4 (pH 4.3) / acetonitrile (75/25)
Flow: 0.2 ml/min
Detection: UV at 260 nm
Temperature: 37 ºC
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| High throughput separation |
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Column: Hydrosphere C18 (3 µm, 12 nm) 50 x 3.0 mm i.d.
Eluent: 10 mM CH3COOH - CH3COONH4 (pH 4.6) / acetonitrile (85/15)
Flow: 1.0 ml/min
Detection: UV at 275 nm
Temperature: 37 ºC
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Hydrosphere C18 is the column of choice within the YMC portfolio for a wide range of different substances. As an almost "universal" selectivity it can be used under standard reversed phase conditions as well as pure aqueous eluents as demonstrated by this collection of applications.
| No ion pair reagent required |
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Catecholamines
Column: Hydrosphere C18 (5 µm, 12 nm) 150 x 4.6 mm i.d.
Eluent: A) 50 mM KH2PO4 - H3PO4 (pH 3.1)
B) 50 mM KH2PO4 - H3PO4 (pH 3.1) / acetonitrile (90/10)
0% B (0-5 min), 0-100% B (5-25 min, linear), 100% B (25-35 min)
Flow: 1.0 ml/min
Detection: UV at 280 nm, 0.64 AUFS
Temperature: 30 ºC
Injection: 10 µl (0.15 - 0.25 mg/ml)
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| Efficient separation of complex substance mixtures |
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Ingredients in an eye drop
Column: Hydrosphere C18 (3 µm, 12 nm) 50 x 4.6 mm i.d.
Eluent: A) 20 mM KH2PO4 - H3PO4 (pH 2.5)
B) acetonitrile
0-60% B (0-10 min), 60% B (10-15 min)
Flow: 1.0 ml/min
Detection: UV at 265nm, 0.128 AUFS
Temperature: 37 ºC
Injection: 5 µl (0.03 - 0.3 mg/ml)
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