The stability properties make YMC-Pack Pro C18 RS an excellent choice for the separation of non-polar structurally related analytes. The extended resistance towards acidic and basic conditions not only widens the possibilities in method development but also provides further selectivities for demanding separations such as LC-MS or combinatorial chemistry of: positional isomers, large hydrophobic molecules, basic and acidic compounds, peptides

Separation of peptides under LC-MS compatible conditions

Column: YMC-Pack Pro C18 RS (5 μm, 8 nm, 150 x 4.6 mm) Eluent: A: water / TFA (100/0.1) B: acetonitrile / TFA (100/0.1) Gradient: 15-35% B (0-15 min) Flow: 1.0 ml/min Detection: UV, 220 nm, 0.32 AUFS Temperature: 37 ºC Injection: 20 μl (0.05 mg/ml)

Separation of acidic and basic drugs under LC-MS compatible conditions

Column: YMC-Pack Pro C18 RS (5 μm, 8 nm, 150 x 4.6 mm) Eluent: 20 mM CH3COONH4-NH3 (pH 8.9) / methanol (35/65) Flow: 1.0 ml/min Detection: UV, 220 nm, 0.64 AUFS Temperature: 30 ºC Injection: 10 μl (0.1 mg/ml, 0.036 μl/ml)

Acidic compounds

Food preservatives Column: YMC-Pack Pro C18 RS (150 x 4.6 mm) Eluent: 20 mM CH3COONa - CH3COOH (pH 4.3) / acetonitrile (80/20) Flow: 1.0 ml/min Detection: UV at 230 nm, 0.26 AUFS Temperature: 37 ºC Injection: 10 μl (0.02 mg/ml)

Food preservatives

Chlorophenol isomers Column: YMC-Pack Pro C18 RS (150 x 4.6 mm) Eluent: acetonitrile / water / acetic acid (30/70/1) Flow: 1.0 ml/min Detection: UV at 230 nm, 0.32 AUFS Temperature: 37 ºC Injection: 10 μl (0.1 - 0.2 mg/ml)

Nicardipine hydrochloride

Column: YMC-Pack Pro C18 RS (150 x 4.6 mm) Eluent: 20 mM KH2PO4-K2HPO4 (pH 6.9) / methanol (25/75) Flow: 1.0 ml/min Detection: UV at 254 nm, 0.32 AUFS Temperature: 37 ºC Injection: 10 μl

For more applications please refer to our “Application Data Collections” or contact us directly.