Preparative LC : Bulk Packing Material : YMC Gel ODS-A

Bulk Packing Material

YMC*Gel ODS-A

fully endcapped C18 material
classic “workhorse” phase
highly versatile ODS phase
for polar to moderately nonpolar pharmaceuticals, organic chemicals, biologicals and natural products

YMC*Gel ODS-A	Specification
Pore size / nm	12	20	30
Particle size / µm	3; 5; 10; 15; 20; 50	3; 5; 10; 15	5; 10; 15
Surface area / m2g-1	330	175	100
Carbon content / %	17	12	7
Recommended pH range	2.0 - 7.5	2.0 - 7.5	2.0 - 7.5
Note: Analytical grades (3 µm and 5 µm) are not available as bulk materials; they are only available in prepacked columns.

General

YMC*Gel ODS-A, YMC’s classic reversed phase packing material, is renowned worldwide because of its excellent performance and reproducibility. This fully scalable C18 phase is successfully used for validated analytical HPLC as well as for large scale preparative purification processes. Over the years YMC*Gel ODS-A has proved its worth in numerous pharmaceutical, biochemical and environmental applications as well as in separations in the field of food technology.

Properties

The production of the base silica for YMC*Gel ODS-A, and the subsequent derivatisation process, are both performed in large bulk batches. Exhaustive endcapping reliably reduces the activity of residual silanol groups and minimises non-specific secondary retention. In addition to standard characterisation methods such as the determination of adsorption isotherms, particle size distribution and carbon content, YMC applies an extensive range of analytical methods to ensure consistent and reproducible selectivity of the reversed phase packings.

The base of YMC*Gel ODS-A is YMC’s high purity silica. This premium silica contains only very low levels of metal contaminants and so prevents significant tailing of sample molecules such as 8-hydroxyquinoline or acetyl acetone, which easily form coordination complexes with metal ions on the silica surface.


Column: YMC-Pack ODS-A (12 nm, 5 µm, 150 x 4.6 mm) Eluent: KH2PO4 (20 mM, pH 7.6)/Methanol = 40/60 Flow: 1.0 ml/min Detection: UV, 254 nm Temperature: 37 °C	Substances: 1. Uracil 2. Acetylacetone 3. 8-Hydroxyquinoline

As coordination functional groups are frequent structural components in pharmaceutical compounds, YMC*Gel ODS-A is an excellent tool for reproducible separations of these compounds without secondary retention or tailing.

YMC*Gel ODS-A is available in 3, 5, 10, 15, 20 and 50 µm particle sizes. As the selectivity is identical throughout the whole particle size range, these phases are ideal for scale-up from analytical to process scale applications.