|
- The desired amount of packing material is weighed in a suitable container, the required amount of slurry solvent is added and the suspension stirred until it is homogeneous. Then the slurry is transferred into the column.
- The column is closed and the piston is raised inside the column at a given jack pressure, expelling excess slurry solvent through the inlet frit. At the end of this process the column is ready for use.
- During column operation, the piston maintains a constant, dynamic pressure on the bed to take up any volume changes caused by settling of the column bed. Formation of voids and channels is therefore prevented. The dynamic aspect of the compression is mandatory for a reproducible column performance.
- Column unpacking is effected under sealed conditions: the piston is moved down and water / solvent is injected from two different inlets into the column. The packing material is resuspended and then removed from the column through a pipe into a container, thereby preventing dispersal of gel into the surrounding workspace.
Recommended packing conditions for YMC gels:
| Stationary phase |
Packing pressure |
Slurry solvent |
Slurry concentration
|
| YMC*Gel Silica (12nm) |
6-7 MPa |
n-Hexane/Ethyl Acetate (1:1) |
40% |
| YMC*Gel ODS-A (12nm) |
8-10 MPa(1) |
Methanol(2) |
30-40% |
| YMC*Gel ODS-AQ (12nm) |
8-10 MPa(1) |
Methanol(2) |
30-40% |
| YMC*Gel Octyl (12nm) |
8-10 MPa(1) |
Methanol(1) |
25-35% |
| YMC*Gel YMCbasic |
8-10 MPa |
Methanol(1) |
25-35% |
(1) for 50 µm particle size: 6-8 MPa recommended
(2) for 15 µm particle size: elutriate in 85 parts of methanol and then add 15 parts of water
|
Start
